Posts Tagged ‘cryoET’
Quantifying the local resolution of cryo-EM density maps | Nature Methods
Wednesday, November 15th, 2017Quantifying the local resolution of #cryoEM density maps
https://www.Nature.com/articles/nmeth.2727 “Theory…based on the following idea: a L Angstrom feature exists at a pt…if a 3D local sinusoid of wavelength L is statistically detectable above noise at that point.”
QT:{{”
We propose a mathematical theory and an efficient algorithm for measuring local resolution that address all of the above limitations. The theory (Online Methods) is based on the following idea: a λ-Å feature exists at a point in the volume if a three-dimensional (3D) local sinusoid of wavelength λ is statistically detectable above noise at that point. A likelihood-ratio hypothesis test of the local sinusoid versus noise can detect this feature at a given P value (typically P = 0.05). We define the local resolution at a point as the smallest λ at which the local sinusoid is detectable, and we account for multiple testing with an FDR procedure.
“}}
Welcome | ScienceHill-CryoEM
Sunday, October 29th, 2017http://sciencehill-cryoem.yale.edu/
cryoem.yale.edu
https://medicine.yale.edu/ccmi/
Visualizing the molecular sociology at the HeLa cell nuclear periphery | Science
Friday, October 27th, 2017http://science.sciencemag.org/content/351/6276/969.long
#Visualizing the molecular sociology [of complexes] at the HeLa cell nuclear periphery, w/ #CryoET at 10 Ang. res. http://science.ScienceMag.org/content/351/6276/969.long
Science. 2016 Feb 26;351(6276):969-72. doi: 10.1126/science.aad8857.
Visualizing the molecular sociology at the HeLa cell nuclear periphery.
Mahamid J1, Pfeffer S2, Schaffer M2, Villa E3, Danev R2, Cuellar LK2, Förster F2, Hyman AA4, Plitzko JM2, Baumeister W1.
Visualizing virus assembly intermediates inside marine cyanobacteria : Nature : Nature Publishing Group
Tuesday, June 16th, 2015Visualizing virus assembly…inside…bacteria
http://www.nature.com/nature/journal/v502/n7473/full/nature12604.html Phase-contrast #cryoET resolves subcellular structures (eg ribosomes)
Cryo-ET determines diff structural snapshots virus during assembly. One tomogram to work out whole pathway.
Wei Dai,
Caroline Fu,
Desislava Raytcheva,
John Flanagan,
Htet A. Khant,
Xiangan Liu,
Ryan H. Rochat,
Cameron Haase-Pettingell,
Jacqueline Piret,
Steve J. Ludtke,
Kuniaki Nagayama,
Michael F. Schmid,
Jonathan A. King
& Wah Chiu
Nature 502, 707–710 (31 October 2013) doi:10.1038/nature12604